Rabbit Polyclonal ATR Antibody

ATR (Ataxia-Telangiectasia and Rad3-related) antibody is a key reagent in studying the ATR protein, a serine/threonine kinase central to the DNA damage response (DDR). ATR, part of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, is activated by single-stranded DNA (ssDNA) generated during replication stress or DNA damage. It coordinates cell cycle checkpoints, DNA repair, and apoptosis to maintain genomic stability. Research involving ATR antibodies focuses on detecting ATR expression, localization, and activation dynamics in cells under stress conditions like UV radiation, chemotherapy, or replication inhibitors. These antibodies are critical in cancer research, as ATR inhibition is explored to sensitize tumor cells to genotoxic therapies. Commercially available ATR antibodies (e.g., from Cell Signaling Technology, Abcam) are validated for techniques like Western blotting, immunofluorescence, and immunoprecipitation. Specificity is ensured through knockout controls, as cross-reactivity with related kinases (e.g., ATM, DNA-PK) must be ruled out. Recent studies also utilize ATR antibodies to investigate its role in diseases beyond cancer, including neurodegenerative disorders and aging. However, challenges remain in distinguishing active (phosphorylated) versus inactive ATR forms, driving the development of phospho-specific antibodies targeting key activation sites like Ser428. Proper validation remains essential for reliable data in DDR studies.