首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人D二聚体(D2D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的D2D含量。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，灵敏度为3.9 ng/mL。适用于基础医学研究，例如通过检测动物模型或体外实验中的D二聚体水平，探究抗凝药物疗效、评估血栓性疾病干预策略，或分析纤溶系统在炎症、肿瘤等病理状态中的作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：D2D
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of human D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 80-96 | ||||
| EDTA plasma (n=4) | 95 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.6952.4982.5972.457 5002.2892.1382.2142.074 2501.5441.4861.5151.375 1250.8130.7830.7980.658 62.50.4150.4010.4080.268 31.20.2790.2740.2770.137 15.60.2170.2110.2140.074 00.1410.1380.140 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human D2D antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled D2D antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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