首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人D-氨基酸氧化酶活化剂(DAOA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DAOA含量。DAOA靶点与精神类疾病相关。研究发现其基因变异会影响神经递质代谢。机制上，它参与调控神经递质如谷氨酸等的水平，影响神经信号传递与大脑功能，有望成为研发治疗精神疾病如精神分裂症等药物的潜在靶点。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，适用于体外研究DAOA在神经生物学机制、疾病模型或药物干预下的表达水平变化，为探索其在中枢神经系统中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：D-amino acid oxidase activator ELISA Kit; DAOA ELISA Kit; DAOA_HUMAN ELISA Kit; G72 transcript ELISA Kit; LG72 ELISA Kit; Protein G72 ELISA Kit; Schizophrenia and bipolar disorder associated protein G72 ELISA Kit; SG72 ELISA Kit
缩写：DAOA
Uniprot No.：P59103
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DAOA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of human DAOA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-101 | ||||
| EDTA plasma (n=4) | 84 | 80-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.6332.7382.6862.51215002.0772.1722.1251.9517501.3531.2821.3181.1443750.6850.6740.6800.506187.50.4250.4360.4310.25793.750.3010.3140.3080.13446.880.2310.2360.2340.06000.1730.1740.174 | ||||||
货期：3-5 working days
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