首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人二胺氧化酶(DAO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DAO含量。人二胺氧化酶（DAO）是一种重要的酶，参与组胺的代谢。研究发现，DAO活性降低会导致组胺在肠内积累，引发胃肠道症状。研究DAO的机制有助于开发治疗过敏和肠道疾病的新方法。试剂盒检测范围为15.6 mIU/mL-1000 mIU/mL，支持科研人员探索DAO在肠道屏障功能、炎症性肠病、食物过敏等领域的调控机制，或作为肠道健康相关研究的生物标志物检测工具。为胃肠功能基础研究、药物开发及病理模型评估提供可靠的技术支持。适用于体外实验研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：D-amino-acid oxidase ELISA Kit; DAAO ELISA Kit; DAMOX ELISA Kit; DAO ELISA Kit; DAO1 ELISA Kit; EC 1.4.3.3 ELISA Kit; MGC35381 ELISA Kit; OXDA ELISA Kit; OXDA_HUMAN ELISA Kit
缩写：DAO
Uniprot No.：P14920
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 mIU/mL-1000 mIU/mL
灵敏度：3.9 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:20 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-102 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected10002.5052.5642.5352.3465001.9952.0042.0001.8112501.3461.3861.3661.1771250.8530.8650.8590.67062.50.5440.5890.5670.37831.250.4450.4580.4520.26315.60.3100.3180.3140.12500.1820.1960.189 | ||||||
货期：3-5 working days
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