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产品描述：人肌萎缩蛋白相关糖蛋白1(DAG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DAG1含量。DAG1（Dystroglycan 1）是一种重要的跨膜蛋白，由α-和β-两个亚基构成，负责细胞外基质配体的结合和与肌营养不良蛋白复合物的连接，维持肌肉细胞稳定性与完整性。其异常表达与肌营养不良症、心肌病等疾病相关，研究其机制有助于理解疾病发病机制和提供治疗思路。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于基础科研领域，如肌肉疾病分子机制研究、肿瘤微环境分析或细胞黏附信号通路探索，能够为体外实验提供可靠的蛋白表达数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：156DAG ELISA Kit; A3a ELISA Kit; AGRNR ELISA Kit; Alpha dystroglycan ELISA Kit; Alpha-DG ELISA Kit; Beta-DG ELISA Kit; Beta-dystroglycan ELISA Kit; DAG ELISA Kit; Dag1 ELISA Kit; DAG1_HUMAN ELISA Kit; Dystroglycan 1 (dystrophin associated glycoprotein 1) ELISA Kit; Dystroglycan ELISA Kit; Dystrophin associated glycoprotein 1 ELISA Kit; Dystrophin-associated glycoprotein 1 ELISA Kit; OTTHUMP00000210857 ELISA Kit; OTTHUMP00000210858 ELISA Kit
缩写：DAG1
Uniprot No.：Q14118
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DAG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of human DAG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-97 | ||||
| EDTA plasma (n=4) | 88 | 81-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.3012.1592.2302.09840001.9341.8681.9011.76920001.5621.5341.5481.41610001.2341.2021.2181.0865000.8250.8450.8350.7032500.5430.5510.5470.4151250.3220.3100.3160.18400.1330.1310.132 | ||||||
货期：3-5 working days
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