Mouse dopamine,DA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08661m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠多巴胺(DA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的DA含量。DA即多巴胺，是重要神经递质。其参与运动控制、情绪调节等生理过程。研究发现DA信号异常与帕金森病、精神分裂症等疾病有关。当前研究围绕DA受体亚型、转运体等机制，旨在开发针对性药物治疗神经及精神类疾病。试剂盒检测范围为15.6 pg/ml-1000 pg/ml.，科研人员可将其应用于神经生物学基础研究，如评估脑区组织匀浆中DA的动态变化，或探究疾病模型中血浆DA水平的调控机制；也可用于药理学实验，分析药物干预对多巴胺能系统的影响，为神经疾病机制解析及潜在治疗策略开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
N/A
缩写：
DA
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
15.6 pg/ml-1000 pg/ml.
灵敏度：
3.9pg/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Neuroscience
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse dopamine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	107
	Range %	103-112
1:2	Average %	95
	Range %	91-105
1:4	Average %	105
	Range %	100-111
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average
1000	0.151	0.144	0.148
500	0.224	0.217	0.221
250	0.341	0.328	0.335
125	0.488	0.519	0.503
62.5	0.699	0.721	0.710
31.25	1.018	1.053	1.035
15.6	1.468	1.384	1.426
0	2.417	2.325	2.371