| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | KRS1; MST2/KRS2; MST1; YSK3; TIIAC |
| WB Predicted band size | 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human STK3/STK4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于STK3/STK4(MST1/MST2)抗体的3篇代表性文献的简要信息，供参考：
---
1. **文献名称**：*The Hippo pathway effector YAP promotes resistance to RAF- and MEK-targeted cancer therapies*
**作者**：Lin L. et al.
**摘要**：该研究通过Western blot和免疫沉淀技术，使用STK3/STK4抗体验证Hippo通路激酶MST1/2的活性对YAP的调控作用，发现YAP激活导致靶向治疗耐药性，提示靶向Hippo通路可能改善癌症治疗效果。
---
2. **文献名称**：*MST1/2 kinases regulate glucose uptake for redox homeostasis in tumor cells*
**作者**：Qin F. et al.
**摘要**：研究利用STK4特异性抗体进行免疫荧光染色，发现MST1/2通过调控葡萄糖转运蛋白GLUT1影响肿瘤细胞代谢，揭示了STK3/STK4在能量应激反应中的新功能。
---
3. **文献名称**：*Regulation of the Hippo-YAP pathway by protease-activated receptors (PARs)*
**作者**：Kim M. et al.
**摘要**：通过siRNA敲低和STK3抗体验证，证明PAR1受体激活抑制MST1/2激酶活性，导致YAP核转位及促增殖基因表达，为GPCR调控Hippo通路提供了机制证据。
---
**注意**：以上文献信息为示例性质，实际引用需以具体数据库(如PubMed、Web of Science)检索结果为准。建议结合研究场景补充抗体品牌(如CST、Abcam等)及实验方法细节。
The STK3 (Serine/Threonine Kinase 3) and STK4 (Serine/Threonine Kinase 4) proteins, also known as MST2 and MST1. respectively, are core components of the Hippo signaling pathway, a conserved regulator of tissue homeostasis, organ size, and apoptosis. These kinases function as tumor suppressors by phosphorylating downstream effectors like LATS1/2. which in turn inhibit YAP/TAZ transcriptional co-activators to control cell proliferation and survival. Dysregulation of STK3/STK4 is implicated in cancer, immune disorders, and neurodegenerative diseases.
Antibodies targeting STK3/STK4 are critical tools for studying Hippo pathway dynamics. They enable detection of protein expression levels, subcellular localization (e.g., cytoplasmic vs. nuclear), and activation status through phosphorylation-specific epitopes (e.g., Thr180/Thr183 in STK3/STK4). Such antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers also utilize them to investigate kinase interactions, pathway crosstalk (e.g., with Wnt or TGF-β signaling), and therapeutic responses in disease models. Due to high sequence homology between STK3 and STK4. some antibodies may cross-react, necessitating careful validation via knockout controls. Recent studies explore STK3/STK4 inhibition as a strategy to enhance tissue regeneration or counteract YAP/TAZ-driven malignancies, further driving demand for specific, high-affinity antibodies in both basic and translational research.
×
