首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人凋亡相关蛋白激酶(DAPK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DAPK1含量。DAPK1（死亡相关蛋白激酶1）是一种钙/钙调蛋白依赖性丝氨酸/苏氨酸激酶，参与细胞信号传导，调节细胞存活、凋亡和自噬。其异常甲基化与多种癌症相关，过表达可诱导细胞凋亡和抑制肿瘤转移。研究DAPK1有助于肿瘤的早期诊断和治疗。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于科研领域研究DAPK在疾病模型中的功能调控、药物干预对凋亡通路的影响，或通过定量分析不同处理条件下细胞及组织内DAPK的动态变化，为分子机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DAK1 ELISA Kit; DAP K1 ELISA Kit; DAP kinase 1 ELISA Kit; DAPK 1 ELISA Kit; DAPK ELISA Kit; DAPK1 ELISA Kit; DAPK1_HUMAN ELISA Kit; Death Associated Protein Kinase 1 ELISA Kit; Death-associated protein kinase 1 ELISA Kit; DKFZp781I035 ELISA Kit
缩写：DAPK1
Uniprot No.：P53355
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DAPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 82-94 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-110 | ||||||
回收率：
| The recovery of human DAPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 90-100 | |||||
| EDTA plasma (n=4) | 97 | 92-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.6092.5342.5722.48710001.6241.7011.6631.5785000.9120.8790.8960.8112500.5310.5120.5220.4371250.2740.2670.2710.18662.50.2460.2350.2410.15631.250.1290.1330.1310.04600.0830.0860.085 | |||||||
货期：3-5 working days
×
