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产品描述：人蛋白水解诱导因子/皮离蛋白(PIF/DCD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、组织匀浆样本中的DCD含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于科研领域中对恶性肿瘤代谢异常、皮肤组织修复机制及相关炎症性疾病的基础研究，特别为探索PIF/DCD在生物体液或细胞分泌模型中的表达规律、评估实验性治疗干预效果提供了可靠的检测工具，广泛应用于分子病理学、药理学及生物标志物筛选等研究方向。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AIDD ELISA Kit; DCD 1 ELISA Kit; dcd ELISA Kit; DCD-1 ELISA Kit; DCD_HUMAN ELISA Kit; Dermcidin ELISA Kit; DSEP ELISA Kit; HCAP ELISA Kit; PIF ELISA Kit; Preproteolysin ELISA Kit
缩写：DCD
Uniprot No.：P81605
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PIF/DCD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human PIF/DCD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.8482.8042.8262.637202.3352.3692.3522.163101.6421.6361.6391.45050.9060.9190.9130.7242.50.5460.5880.5670.3781.250.3430.3560.3500.1610.6250.2520.2630.2580.06900.1920.1860.189 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human DCD antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled DCD antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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