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Human dopamine-β-hydroxylase,DBH ELISA Kit

  • 中文名： 人多巴胺-β羟化酶(DBH)ELISA Kit
货号: IPD-E09653h
Price： ￥2500
数量：
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  • 产品描述：人多巴胺-β羟化酶(DBH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DBH含量。DBH即多巴胺β-羟化酶，是去甲肾上腺素合成的关键酶。其在心血管调节、神经递质代谢等方面至关重要。研究主要围绕其活性调节机制、与疾病关系等，如在高血压、神经精神疾病中作用机制探索，对理解疾病病理及开发治疗药物有重要意义。试剂盒检测范围为2.5 ng/mL-500 ng/mL，适用于神经生物学、药理学等领域的基础研究，如神经退行性疾病模型、精神分裂症动物模型、高血压相关机制研究等场景中DBH表达水平的动态监测，或通过检测外周血样本评估交感神经活性变化，为探索交感神经活性调控及相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：dbh ELISA Kit; DBM ELISA Kit; Dopamine beta hydroxylase ELISA Kit; Dopamine beta monooxygenase ELISA Kit; Dopamine beta-hydroxylase (dopamine beta-monooxygenase) ELISA Kit; Dopamine beta-monooxygenase ELISA Kit; DOPO_HUMAN ELISA Kit; OTTHUMP00000022501 ELISA Kit; Soluble dopamine beta-hydroxylase ELISA Kit

  • 缩写：DBH

  • Uniprot No.：P09172

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：2.5 ng/mL-500 ng/mL

  • 灵敏度：1.25 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Metabolism

  • 测定原理：quantitative

  • 测定方法：Competitive

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human DBH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %90
    Range %82-96
    1:2Average %95
    Range %91-100
    1:4Average %98
    Range %91-105
    1:8Average %93
    Range %84-99

  • 回收率：

    The recovery of human DBH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9490-99
    EDTA plasma (n=4)9790-104














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average  01.9581.9631.961  2.51.3111.3211.316  100.8120.8360.824  400.5160.5270.522  1250.3190.3240.322  5000.1860.1890.188  






  • 货期：3-5 working days

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