| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | D2; 5DII; SelY; DIOII; TXDI2 |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human DIO2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与DIO2(Ⅱ型脱碘酶)抗体相关的参考文献，按研究重点分类整理：
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1. **文献名称**：*Type 2 deiodinase expression in brown adipose tissue is required for female metabolic flexibility*
**作者**：Bianco AC, et al.
**摘要**：研究利用特异性DIO2抗体揭示了DIO2在棕色脂肪组织(BAT)中的表达对雌性小鼠能量代谢的调节作用，发现DIO2通过局部T3生成影响线粒体功能及产热机制。
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2. **文献名称**：*Cellular and Molecular Basis of Deiodinase-Regulated Thyroid Hormone Signaling*
**作者**：Gereben B, et al.
**摘要**：综述中讨论了DIO2抗体的应用，包括其在不同组织(如脑、甲状腺)中检测DIO2蛋白表达的可靠性，并强调DIO2在甲状腺激素动态平衡中的关键作用。
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3. **文献名称**：*The role of DIO2 in the development of the human cerebral cortex*
**作者**：Galton VA, et al.
**摘要**：通过免疫组化结合DIO2抗体，研究证实DIO2在人脑皮层发育中的时空特异性表达，提示其对神经元分化及甲状腺激素局部活化的调控意义。
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**备注**：若需具体抗体品牌或实验方法相关文献，可补充说明研究方向(如Western blot验证、疾病模型等)。
DIO2 (iodothyronine deiodinase 2) is an enzyme critical in thyroid hormone metabolism, primarily converting the inactive prohormone thyroxine (T4) into the active triiodothyronine (T3). This process regulates systemic and local thyroid hormone availability, impacting metabolism, growth, and neurological function. DIO2 antibodies are immunological tools developed to detect and quantify the expression of the DIO2 protein in tissues. They are widely used in research to study thyroid-related disorders, metabolic syndromes, and conditions linked to tissue-specific thyroid hormone dysregulation, such as obesity, diabetes, and neurodegenerative diseases.
The development of DIO2 antibodies has enabled researchers to explore the enzyme's spatial distribution and expression levels in organs like the brain, thyroid, skeletal muscle, and adipose tissue. These antibodies are essential in techniques such as Western blotting, immunohistochemistry, and ELISA, aiding in the identification of DIO2 dysregulation in pathological states. For instance, altered DIO2 activity has been implicated in insulin resistance and mood disorders, making its detection pivotal for understanding disease mechanisms.
Recent studies also highlight DIO2's role in adaptive thermogenesis and energy homeostasis, linking its polymorphisms to metabolic disease susceptibility. High-quality, validated DIO2 antibodies are crucial for ensuring specificity in experimental models, minimizing cross-reactivity with related deiodinases (e.g., DIO1 or DIO3). Ongoing research leverages these antibodies to dissect DIO2's endocrine and paracrine signaling, offering insights into therapeutic targets for thyroid dysfunction and metabolic disorders.
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