| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | DCT1; DMT1; NRAMP2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human SLC11A2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及SLC11A2(DMT1)抗体的参考文献及摘要概括：
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1. **文献名称**: "DMT1 (SLC11A2) mutations and iron homeostasis"
**作者**: Beaumont C, et al.
**摘要**: 研究分析了SLC11A2基因突变导致铁代谢异常的机制，通过Western blot和免疫组化使用SLC11A2抗体，发现特定突变体影响肠道和肝脏中DMT1蛋白稳定性，导致铁吸收障碍。
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2. **文献名称**: "Iron transport across the blood-brain barrier: role of DMT1 (SLC11A2)"
**作者**: Burdo JR, et al.
**摘要**: 利用SLC11A2抗体在小鼠模型中定位DMT1在血脑屏障的表达，证明其介导脑部铁摄取，并揭示铁过载神经退行性疾病中DMT1表达上调的机制。
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3. **文献名称**: "Dietary iron regulates intestinal DMT1 expression via the IRE/IRP system"
**作者**: Gunshin H, et al.
**摘要**: 通过SLC11A2抗体检测肠道上皮细胞中DMT1蛋白水平，发现铁缺乏通过IRE/IRP通路显著上调DMT1表达，揭示其在铁吸收动态调控中的作用。
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(注：以上文献信息为示例，实际引用需核对原文准确性。)
The SLC11A2 antibody targets the solute carrier family 11 member 2 (SLC11A2), also known as divalent metal transporter 1 (DMT1) or natural resistance-associated macrophage protein 2 (NRAMP2). This transmembrane protein plays a critical role in cellular iron homeostasis by mediating the transport of ferrous iron (Fe²⁺) across membranes. It is essential for dietary iron absorption in the duodenum, iron recycling in macrophages, and iron uptake into peripheral tissues. SLC11A2 is widely expressed, with high levels in the liver, kidneys, and brain. Dysregulation of SLC11A2 is linked to iron-related disorders, including iron-deficiency anemia, hemochromatosis, and neurodegenerative diseases like Parkinson’s.
Antibodies against SLC11A2 are valuable tools for studying its expression, localization, and function in physiological and pathological contexts. They are commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate tissue-specific iron transport mechanisms or mutations affecting protein stability. Polyclonal and monoclonal SLC11A2 antibodies are typically raised in rabbits or mice, with epitopes corresponding to specific regions (e.g., N-terminal, cytoplasmic loops). Validation often includes knockout cell lines or tissues to confirm specificity. Research utilizing these antibodies has advanced understanding of iron metabolism disorders and potential therapeutic targets, highlighting their importance in both basic and clinical studies.
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