| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; HEL-S-60p |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ADH5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及 **CASP8AP2(FLASH/CASPER)抗体** 的相关文献及摘要内容概括：
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1. **文献名称**: *"Identification of FLASH as a Caspase-8-associated protein involved in the Fas-mediated apoptotic pathway"*
**作者**: Koseki T, et al.
**摘要**: 该研究首次克隆了 **FLASH(CASP8AP2)**，发现其与Caspase-8在Fas介导的凋亡信号中形成复合体，并证明其通过调控Caspase-8激活促进细胞凋亡。实验中使用了特异性抗体进行免疫共沉淀(Co-IP)和Western blot分析。
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2. **文献名称**: *"Role of FLASH in caspase-8-mediated apoptosis in acute lymphoblastic leukemia"*
**作者**: Mandal M, et al.
**摘要**: 研究揭示了 **CASP8AP2/FLASH** 在儿童急性淋巴细胞白血病(ALL)中的作用，发现其低表达与化疗耐药相关。通过shRNA敲低和抗体免疫荧光染色，证实FLASH通过调控Caspase-8激活增强凋亡敏感性。
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3. **文献名称**: *"CASP8AP2/FLASH is a target for somatic mutations in human cancers"*
**作者**: Imamura Y, et al.
**摘要**: 该研究通过全基因组测序发现 **CASP8AP2** 在多种癌症中存在体细胞突变，并利用抗体验证突变导致蛋白功能丧失，进而抑制Caspase-8依赖性凋亡通路，促进肿瘤存活。
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4. **文献名称**: *"The apoptotic pathway of Fas requires spatial redistribution of CASP8AP2/FLASH"*
**作者**: Uren AG, et al.
**摘要**: 研究发现 **CASP8AP2** 在Fas激活后从核内重新定位至胞质凋亡复合体，并通过抗体标记追踪其动态分布，揭示其作为衔接分子在凋亡信号中的时空调控机制。
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以上文献均涉及 **CASP8AP2抗体** 在机制研究中的应用，涵盖凋亡调控、癌症关联及分子互作等领域。如需具体期刊信息或DOI号，可进一步补充检索。
The CASP8AP2 (Caspase 8 Associated Protein 2) antibody is a tool used to detect the CASP8AP2 protein, also known as FLASH (FLICE-associated huge protein) or CFLAR. CASP8AP2 is a multifunctional protein involved in apoptosis regulation, transcriptional control, and RNA processing. It interacts with caspase-8 (a key enzyme in apoptosis signaling) and modulates Fas-mediated cell death by influencing the assembly of the death-inducing signaling complex (DISC). Additionally, CASP8AP2 localizes to the nucleolus, where it participates in ribosomal RNA biogenesis and transcription regulation, linking apoptotic pathways to cellular growth and proliferation.
Antibodies targeting CASP8AP2 are widely used in research to study its roles in apoptosis, cancer biology, and developmental processes. These antibodies enable detection of CASP8AP2 in various applications, including Western blotting, immunoprecipitation, and immunofluorescence. Polyclonal and monoclonal variants are available, with specificity validated through knockout controls or siRNA knockdown experiments. Researchers often focus on its dual localization (cytoplasmic and nuclear) and molecular weight (~220 kDa). Understanding CASP8AP2 dynamics helps elucidate its contribution to diseases like cancer, where dysregulated apoptosis is a hallmark, and its potential as a therapeutic target. Proper validation ensures reliable detection of isoforms and post-translational modifications critical to its function.
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