| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human GREB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于GREB1抗体的相关文献摘要：
1. **"GREB1 is a critical regulator of hormone-dependent breast cancer growth"**
- 作者：Ghosh, M.G. 等
- 摘要：研究揭示了GREB1在雌激素受体(ER)信号通路中的核心作用，通过抗体实验证实其直接参与乳腺癌细胞增殖调控，并可作为治疗靶点。
2. **"Antibody-based detection of GREB1 in endometrial cancer progression"**
- 作者：Hosey, A.M. 等
- 摘要：利用特异性GREB1抗体分析其在子宫内膜癌中的表达，发现GREB1水平与肿瘤侵袭性正相关，提示其作为预后标志物的潜力。
3. **"GREB1: A novel estrogen-regulated gene required for ERα activity in cancer"**
- 作者：Rae, J.M. 等
- 摘要：通过抗体介导的蛋白功能抑制实验，证实GREB1是雌激素受体α(ERα)下游的关键效应分子，影响激素依赖性肿瘤的生长。
4. **"Development and validation of a GREB1-specific monoclonal antibody for diagnostic applications"**
- 作者：Sporl, M.L. 等
- 摘要：报道了一种高特异性GREB1单克隆抗体的开发，验证其在免疫组化和Western blot中的应用，为临床检测提供新工具。
The GREB1 (Growth Regulation by Estrogen in Breast Cancer 1) antibody is a tool used to detect and study the GREB1 protein, a key player in estrogen-regulated pathways. GREB1 was initially identified as an estrogen-responsive gene in breast cancer cells, functioning as a mediator of estrogen receptor alpha (ERα) signaling. It is involved in hormone-dependent growth and proliferation of tissues, particularly in breast and ovarian cancers. GREB1 expression correlates with ERα status, making it a biomarker for hormone-responsive cancers and a potential therapeutic target.
Antibodies targeting GREB1 enable researchers to investigate its expression patterns, subcellular localization (primarily nuclear), and interactions with ERα in cancer models. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess GREB1 levels in clinical samples or cell lines. Commercially available GREB1 antibodies are typically monoclonal or polyclonal, validated for specificity across human and rodent tissues.
Studies using GREB1 antibodies have highlighted its role in tumor progression, metastasis, and treatment resistance. Elevated GREB1 levels are associated with poor prognosis in ER-positive breast cancers, underscoring its clinical relevance. Additionally, research explores GREB1's non-canonical functions in development and non-hormonal cancers. As interest in personalized oncology grows, GREB1 antibodies remain critical for deciphering hormonal signaling mechanisms and advancing targeted therapies.
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