| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | H2A/m; H2AFM; HIST1H2AB |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human H2AC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于H2AC4抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **文献名称**: "Histone H2A variants in nucleosome stability and chromatin assembly"
**作者**: Smith, E.M., et al.
**摘要**: 研究分析了H2A组蛋白变体(包括H2AC4)在染色质动态中的作用，通过特异性抗体检测H2AC4在细胞周期中的定位变化，发现其参与DNA损伤修复过程，并验证了抗体在ChIP-seq中的适用性。
2. **文献名称**: "Epigenetic regulation by H2A.Z and H2AC4 in cancer progression"
**作者**: Wang, L., & Zhang, Y.
**摘要**: 文章利用H2AC4抗体探究其在乳腺癌中的表达模式，发现H2AC4与H2A.Z协同调控致癌基因的表观遗传沉默，并通过Western blot和免疫组化证实抗体特异性。
3. **文献名称**: "Antibody validation for histone post-translational modifications: a case study of H2AC4 phosphorylation"
**作者**: Johnson, R., et al.
**摘要**: 该研究系统评估了针对H2AC4磷酸化位点的抗体特异性，通过质谱和敲除实验验证抗体可靠性，为研究H2AC4在细胞应激响应中的功能提供工具支持。
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注：以上文献为示例，实际引用时需核实具体文献信息及数据库(如PubMed、Web of Science)中的真实来源。如需具体文章，建议检索关键词“H2AC4 antibody”或“H2A histone variant”。
The H2AC4 antibody targets histone H2A type 4 (H2AC4), a core component of nucleosomes responsible for DNA packaging and epigenetic regulation. As part of the H2A histone family, H2AC4 plays a critical role in maintaining chromatin structure and modulating gene expression through post-translational modifications (e.g., acetylation, phosphorylation). Antibodies against H2AC4 are widely used to study its localization, expression, and interactions in cellular processes such as DNA repair, replication, and transcriptional regulation.
These antibodies are essential tools in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to investigate histone dynamics in developmental biology, cancer research, and epigenetic studies. For example, altered H2AC4 expression or modification patterns have been linked to diseases, including cancers, where chromatin remodeling is dysregulated. Most H2AC4 antibodies are raised in rabbits or mice using peptide antigens corresponding to conserved regions of the protein, and specificity is validated via knockout controls or competitive assays. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to other H2A variants (e.g., H2AZ or H2AX) to ensure accurate experimental outcomes. Understanding H2AC4's role through such antibodies contributes to insights into chromatin-related mechanisms and therapeutic targeting.
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