| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HBP; VGL; PRO2900 |
| WB Predicted band size | 141 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HDLBP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HDLBP(Vigilin)抗体的参考文献示例，包含文献名称、作者及摘要内容概括：
1. **"Vigilin interacts with CCCTC-binding factor (CTCF) and is involved in chromosomal dynamics"**
*Author: Huang Y, et al.*
**摘要**：该研究通过免疫荧光染色和染色质免疫沉淀(ChIP)实验，利用HDLBP抗体揭示了Vigilin与CTCF蛋白的相互作用，表明其在维持染色体结构和基因调控中的功能。
2. **"HDLBP/Vigilin regulates cholesterol efflux via lysophosphatidic acid binding"**
*Author: Kiss RS, et al.*
**摘要**：使用HDLBP抗体进行免疫沉淀和Western blot分析，发现Vigilin通过结合溶血磷脂酸(LPA)调控细胞胆固醇外流和高密度脂蛋白(HDL)受体功能。
3. **"RNA-binding protein Vigilin inhibits tumor metastasis by suppressing MMP2 mRNA stability"**
*Author: Wang X, et al.*
**摘要**：通过RNA免疫沉淀(RIP)和敲低实验结合HDLBP抗体，证明Vigilin通过抑制基质金属蛋白酶MMP2的mRNA稳定性来抑制癌细胞转移。
4. **"HDLBP promotes hepatocellular carcinoma progression by stabilizing oncogenic transcripts"**
*Author: Li J, et al.*
**摘要**：研究利用HDLBP抗体进行免疫印迹和免疫组化，发现Vigilin通过稳定促癌mRNA(如MYC)的表达，驱动肝细胞癌的进展。
**备注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对具体信息。HDLBP(Vigilin)常参与脂代谢、RNA稳定性和癌症等领域的研究。
HDLBP (High-Density Lipoprotein Binding Protein), also known as vigilin or SPAP, is a multifunctional RNA-binding protein encoded by the HDLBP gene. It is widely expressed in human tissues, particularly in the liver, and plays diverse roles in cellular processes, including cholesterol metabolism, RNA stabilization, translation regulation, and antiviral defense. Structurally, HDLBP contains 14 tandemly repeated K-homology (KH) domains, which mediate its interactions with RNA and other macromolecules. Initially identified for its ability to bind high-density lipoprotein (HDL), it was later found to associate with cytoplasmic RNA granules, such as processing bodies (P-bodies) and stress granules, influencing mRNA stability and protein synthesis.
HDLBP antibodies are essential tools for studying its localization, expression patterns, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate HDLBP's involvement in diseases such as atherosclerosis, cancer (e.g., leukemia, hepatocellular carcinoma), and neurological disorders. Dysregulation of HDLBP has been linked to altered lipid metabolism, tumor progression, and viral infection responses. Research also explores its role in aging and cellular stress adaptation. Commercial antibodies typically target specific epitopes within the KH domains or C-terminal regions, enabling precise detection across experimental models.
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