| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HACE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HACE1抗体的3篇参考文献示例(文献信息为模拟，仅供参考)：
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1. **文献名称**：*HACE1 suppresses tumor progression by targeting Rac1 for ubiquitin-mediated degradation*
**作者**：Li, X., Zhang, Y., & Wei, H.
**摘要**：本研究揭示HACE1通过泛素化降解Rac1蛋白抑制肿瘤转移。实验中采用HACE1特异性抗体进行Western blot和免疫组化，证实HACE1在多种癌症组织中低表达，并与其泛素连接酶活性相关。
2. **文献名称**：*HACE1 deficiency disrupts mitochondrial function via modulating ROS signaling*
**作者**：Park, S., Kim, J., & Lee, M.
**摘要**：文章探讨HACE1缺失导致线粒体功能异常的机制。通过HACE1抗体进行免疫沉淀和质谱分析，发现其与氧化应激相关蛋白相互作用，并利用该抗体验证小鼠模型中HACE1的组织特异性表达模式。
3. **文献名称**：*A novel HACE1 antibody validates its role in neuronal development and autophagy regulation*
**作者**：Garcia, R., et al.
**摘要**：开发了一种新型高特异性HACE1单克隆抗体，应用于免疫荧光和流式细胞术。实验证明HACE1在神经元发育中调控自噬通路，抗体验证了其在脑组织和小胶质细胞中的亚细胞定位。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“HACE1 antibody”、“HACE1 ubiquitin ligase”或结合具体研究领域(如癌症、神经科学)进一步筛选。
The HACE1 (HECT Domain and Ankyrin Repeat Containing E3 Ubiquitin Protein Ligase 1) antibody is a research tool used to study the HACE1 protein, an E3 ubiquitin ligase involved in regulating cellular processes like protein degradation, signal transduction, and stress responses. HACE1 facilitates the attachment of ubiquitin molecules to specific target proteins, marking them for proteasomal degradation. It is recognized for its tumor-suppressive role, as its loss or mutation is linked to various cancers, including neuroblastoma, colorectal, and breast cancers. HACE1 also regulates redox homeostasis by targeting Rac1 for ubiquitination, thereby mitigating oxidative stress.
Antibodies against HACE1 enable the detection and quantification of the protein in experimental models (e.g., Western blot, immunohistochemistry) to explore its expression patterns, functional interactions, and pathological relevance. Research using HACE1 antibodies has highlighted its involvement in autophagy, apoptosis, and immune regulation. Dysregulation of HACE1 is associated with neurodegenerative diseases and metabolic disorders, underscoring its broad physiological impact. These antibodies are critical for unraveling HACE1's molecular mechanisms and potential therapeutic applications in diseases marked by ubiquitination pathway disruptions.
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