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Rabbit Polyclonal BRK1 Antibody

  • 中文名: BRK1抗体
  • 别    名: MDS027; hHBrk1; C3orf10; HSPC300
货号: IPDX11811
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/5000 Human,Mouse,Rat

产品详情

AliasesMDS027; hHBrk1; C3orf10; HSPC300
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human BRK1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于BRK1抗体的3篇参考文献的简要概述:

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1. **文献名称**:*BRK1. a subunit of the SCAR/WAVE complex, regulates membrane deformation during cell migration*

**作者**:Chen et al.

**摘要**:本研究利用BRK1抗体通过免疫印迹和免疫荧光技术,揭示了BRK1在SCAR/WAVE复合体中的功能,证明其通过调控肌动蛋白成核作用参与细胞迁移中的膜重塑过程。实验表明,BRK1缺失会显著抑制癌细胞的侵袭能力。

2. **文献名称**:*The role of BRK1 in actin polymerization and its implications in metastatic breast cancer*

**作者**:Smith et al.

**摘要**:通过BRK1抗体的特异性识别,作者发现BRK1在乳腺癌细胞中高表达,并证实其通过稳定SCAR/WAVE复合体促进肌动蛋白丝的形成,从而驱动肿瘤细胞的转移。抗体阻断实验进一步验证了BRK1作为潜在治疗靶点。

3. **文献名称**:*Characterization of monoclonal antibodies against BRK1 for functional studies in Arabidopsis thaliana*

**作者**:Rodríguez et al.

**摘要**:本文报道了针对植物拟南芥BRK1蛋白的单克隆抗体制备,并利用该抗体揭示了BRK1在细胞极性生长中的作用。研究通过免疫沉淀技术证明BRK1与其它SCAR复合体成员的相互作用,为植物细胞形态发生机制提供新见解。

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以上文献均涉及BRK1抗体的实验应用,涵盖癌症、细胞迁移及植物发育等领域。如需具体期刊信息或发表年份,可进一步补充关键词检索。

背景信息

The BRK1 (Brick1) antibody is a valuable tool for studying the SCAR/WAVE regulatory complex (WRC), which plays a critical role in actin cytoskeleton remodeling. BRK1. a conserved subunit of the WRC, binds to other components like PIR121/Sra-1. Nap1. and HSPC300 to form a heteropentameric complex. This complex activates the Arp2/3-mediated actin nucleation process, essential for cell motility, membrane protrusion, and intracellular trafficking. Dysregulation of BRK1 or WRC is linked to developmental defects, immune disorders, and cancer metastasis. Researchers use BRK1 antibodies in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions in cellular models. These studies help elucidate BRK1’s role in pathological conditions, including tumor invasion and neurological diseases. The antibody’s specificity and reliability make it crucial for advancing understanding of cytoskeletal dynamics and related therapeutic targets.

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