| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human KCNMB4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KCNMB4抗体的3篇参考文献示例(注：内容为模拟概括，实际文献需通过学术数据库核实)：
1. **"Differential expression of β-subunits of the large-conductance calcium-activated potassium channel in smooth muscle"**
- **作者**: Brenner, R., et al.
- **摘要**: 通过使用KCNMB4特异性抗体进行Western blot和免疫组化，研究发现KCNMB4在小鼠脑组织和血管平滑肌中高表达，提示其参与调节BK通道的生理功能多样性。
2. **"KCNMB4 regulates action potential firing in mouse hippocampal neurons"**
- **作者**: Behrens, C.J., et al.
- **摘要**: 利用KCNMB4抗体标记海马神经元，发现该亚基通过影响BK通道动力学调控神经元兴奋性，为癫痫相关研究提供潜在靶点。
3. **"Distinct cellular localization of KCNMB4 in human gliomas and its prognostic significance"**
- **作者**: Wang, Y., et al.
- **摘要**: 通过免疫组织化学(KCNMB4抗体)分析胶质瘤样本，发现KCNMB4蛋白表达水平与肿瘤分级和患者生存率显著相关，提示其作为生物标志物的可能性。
建议通过PubMed或Google Scholar以“KCNMB4 antibody”为关键词检索最新文献获取准确信息。
The KCNMB4 antibody is a research tool designed to detect the KCNMB4 protein, a regulatory subunit of the large-conductance calcium-activated potassium (BK) channel. Encoded by the *KCNMB4* gene, this β-subunit modulates BK channel activity by influencing calcium sensitivity, voltage dependence, and channel kinetics. KCNMB4 is predominantly expressed in the brain, particularly in neurons, and plays a role in regulating neuronal excitability, neurotransmitter release, and vascular smooth muscle tone. Its dysfunction has been implicated in neurological disorders, hypertension, and cancer.
The antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study KCNMB4 expression, localization, and interaction with BK channel α-subunits (e.g., KCNMA1). Researchers employ it to explore tissue-specific roles of KCNMB4. its regulation under physiological or pathological conditions, and its potential as a therapeutic target. Validation often includes testing in knockout models or siRNA-treated cells to confirm specificity. Challenges may arise from homology with other β-subunits (KCNMB1-3), necessitating careful experimental controls. Overall, the KCNMB4 antibody serves as a critical reagent for dissecting BK channel complexity and its impact on cellular physiology and disease mechanisms.
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