| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | RDL |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human LEO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LEO1抗体的3篇示例文献(内容为模拟概括，非真实文献)：
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1. **文献名称**: "LEO1. a Component of the PAF1 Complex, Regulates Histone H3K4 Methylation in Yeast"
**作者**: Smith J. et al.
**摘要**: 研究揭示了LEO1作为PAF1复合体的关键成员，通过与RNA聚合酶II结合调控组蛋白H3K4甲基化，影响基因转录延伸和染色质修饰过程。研究使用LEO1抗体进行ChIP-seq分析，证实其在转录起始和延伸中的双重作用。
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2. **文献名称**: "LEO1 Antibody-Based Detection of Dysregulated PAF1 Complex in Colorectal Cancer"
**作者**: Chen L. et al.
**摘要**: 通过LEO1抗体的免疫组化分析，发现PAF1复合体(含LEO1)在结直肠癌组织中异常高表达，且与患者预后不良相关。研究表明LEO1可能通过激活致癌基因转录促进肿瘤进展。
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3. **文献名称**: "Structural and Functional Analysis of LEO1 in Mammalian mRNA Processing"
**作者**: Tanaka R. et al.
**摘要**: 利用LEO1抗体的免疫共沉淀技术，发现哺乳动物LEO1不仅参与转录延伸，还与mRNA加尾因子相互作用，调控mRNA稳定性及核质转运，揭示其在转录后调控中的新功能。
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注：以上文献为示例，实际文献需通过PubMed/Google Scholar等平台检索。LEO1相关研究多围绕其在PAF1复合体中的功能及表观遗传调控机制展开。
The LEO1 (Left Open Reading Frame 1) antibody is a tool used to study the LEO1 protein, a key component of the RNA polymerase II-associated factor 1 (PAF1) complex. This complex is evolutionarily conserved and plays a critical role in transcriptional elongation, histone modification, and chromatin remodeling. LEO1. along with other PAF1 members (e.g., PAF1. CTR9. CDC73. RTF1), interacts with RNA polymerase II to regulate gene expression by facilitating the recruitment of histone-modifying enzymes, such as those involved in H2B ubiquitination and H3K4 methylation. These processes influence transcriptional fidelity, mRNA stability, and epigenetic regulation.
LEO1 is implicated in diverse cellular functions, including stem cell maintenance, development, and oncogenesis. Dysregulation of the PAF1 complex has been linked to cancers, developmental disorders, and viral replication. The LEO1 antibody is widely used in research to investigate its role in these pathways through techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence. It helps identify LEO1-protein interactions, map genomic binding sites, and assess its expression levels in disease models. As a reagent, the antibody’s specificity and reliability are critical for elucidating mechanistic insights into transcriptional regulation and potential therapeutic targets.
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