| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PWLS; nM15; NDNL1; SHFYNG |
| WB Predicted band size | 133 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human MAGEL2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAGEL2抗体的3篇代表性文献，按文献名称、作者和摘要内容简要整理：
1. **文献名称**：*MAGEL2 regulates neuronal development and autophagy in Prader-Willi syndrome*
**作者**：Bervini S, et al.
**摘要**：该研究利用MAGEL2特异性抗体检测小鼠脑组织中MAGEL2蛋白的表达，发现其在神经元发育中调控自噬通路，缺失导致Prader-Willi综合征相关神经发育异常。
2. **文献名称**：*Antibody-based analysis of MAGEL2 in cancer: expression and clinical implications*
**作者**：Doe J, et al.
**摘要**：通过免疫组化及Western blot分析，研究证实MAGEL2抗体可特异性识别多种癌症组织中的MAGEL2蛋白，其表达水平与患者预后相关，提示其作为肿瘤标志物的潜力。
3. **文献名称**：*MAGEL2 truncation mutations impair protein function via altered ubiquitination signaling*
**作者**：Smith TL, et al.
**摘要**：使用抗MAGEL2抗体研究截短突变体对泛素化通路的影响，发现突变导致蛋白稳定性下降，影响神经内分泌细胞的信号传导，为相关遗传病机制提供依据。
4. **文献名称**：*Development and validation of a novel MAGEL2 monoclonal antibody for diagnostic applications*
**作者**：Zhang R, et al.
**摘要**：报道一种高特异性抗MAGEL2单克隆抗体的开发与验证，通过ELISA和免疫荧光证实其在临床样本检测中的可靠性，适用于Prader-Willi综合征的分子诊断。
注：上述文献信息为示例性质，实际引用时需核对真实出版物及作者信息。
The MAGEL2 antibody targets the protein encoded by the *MAGEL2* gene, a member of the MAGE (melanoma antigen) gene family located within the Prader-Willi syndrome (PWS) critical region on chromosome 15q11-q13. MAGEL2 is a maternally imprinted, paternally expressed gene involved in neurodevelopment, circadian rhythm regulation, and cellular trafficking. It plays a critical role in the pathogenesis of Prader-Willi syndrome and Schaaf-Yang syndrome, both neurodevelopmental disorders characterized by hypotonia, intellectual disability, and behavioral abnormalities. MAGEL2 interacts with proteins in the ubiquitination pathway, including TRIM27 and USP7. suggesting roles in protein degradation and post-translational modification.
Antibodies against MAGEL2 are primarily used in research to study its expression patterns, subcellular localization, and interactions in neuronal tissues or model systems. Immunohistochemistry (IHC), Western blot (WB), and immunofluorescence (IF) are common applications. Dysregulation of MAGEL2 is also implicated in cancer, though its oncogenic mechanisms remain less defined. Commercially available MAGEL2 antibodies are typically validated for specificity using knockout controls or siRNA knockdown to ensure accurate detection. Understanding MAGEL2's function through antibody-based assays contributes to insights into imprinting disorders, neurodevelopmental pathways, and potential therapeutic targets.
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