| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PAB1; PABP; PABP1; PABPC2; PABPL1 |
| WB Predicted band size | 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PABPC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TBX5抗体的示例参考文献(信息为示例性质，非真实文献)：
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1. **文献名称**: *TBX5 regulates early cardiac development through transcriptional activation of target genes*
**作者**: Smith A, et al.
**摘要**: 本研究利用TBX5特异性抗体，通过免疫组化和Western blot分析，揭示了TBX5在小鼠胚胎心脏发育中的表达模式及其对下游基因(如Nkx2.5)的调控作用。
2. **文献名称**: *Immunolocalization of TBX5 in limb bud morphogenesis*
**作者**: Chen L, et al.
**摘要**: 通过抗TBX5抗体的免疫荧光染色，作者发现TBX5蛋白在鸡胚肢芽发育中的动态分布，提示其在前肢形成中的关键作用。
3. **文献名称**: *Dysregulation of TBX5 in Holt-Oram syndrome: Insights from patient-derived cells*
**作者**: Tanaka M, et al.
**摘要**: 研究使用TBX5抗体进行染色质免疫沉淀(ChIP)，发现Holt-Oram综合征患者的TBX5突变导致与心脏发育相关基因的结合能力下降。
4. **文献名称**: *Comparative analysis of TBX5 antibody specificity across species*
**作者**: Kumar R, et al.
**摘要**: 文章系统评估了多种市售TBX5抗体的交叉反应性，为跨物种研究(如斑马鱼、小鼠、人类)中的抗体选择提供了实验依据。
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**注意**：以上文献信息为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索确认。真实研究中，Bruneau实验室(如Nature 1999. PMID:9924026)及Ghosh等人(Development 2009)可能涉及TBX5抗体应用。
The TBX5 antibody is a crucial tool in studying the TBX5 protein, a member of the T-box transcription factor family essential for embryonic development, particularly in heart and limb formation. TBX5 plays a pivotal role in regulating gene expression during organogenesis, with mutations linked to congenital disorders like Holt-Oram syndrome, characterized by heart defects and upper limb abnormalities. The antibody is widely used in research to detect TBX5 expression in tissues, investigate its functional mechanisms, and explore its interactions with other developmental regulators (e.g., GATA4. NKX2-5).
Developed using immunogenic peptides or recombinant proteins, TBX5 antibodies are validated for specificity in assays such as Western blot, immunohistochemistry, and immunofluorescence. They enable visualization of TBX5 localization in nuclei, confirming its role as a DNA-binding transcription factor. Commercial availability from biotech vendors has facilitated studies on TBX5's involvement in cardiac chamber specification, conduction system development, and disease modeling. Recent applications extend to stem cell differentiation and regenerative medicine, where TBX5 guides in vitro cardiomyocyte programming.
Despite its utility, researchers must verify antibody performance across species and experimental conditions due to potential cross-reactivity with related T-box proteins. Proper controls ensure accurate interpretation of TBX5's spatiotemporal expression patterns in developmental and pathological contexts.
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