| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MIG6; RALT; MIG-6; GENE-33 |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ERRFI1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及ERRFI1抗体的代表性文献及其摘要概括：
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1. **文献名称**: "MIG6 negatively regulates EGFR signaling and acts as a tumor suppressor in lung cancer"
**作者**: Zhang YW, et al.
**摘要**: 本研究利用ERRFI1(MIG6)特异性抗体，通过免疫组化分析发现ERRFI1在非小细胞肺癌组织中表达下调。实验证实ERRFI1通过抑制EGFR信号通路抑制肿瘤生长，抗体检测结果显示其表达缺失与患者预后不良相关。
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2. **文献名称**: "RALT interacts with the EGFR/ErbB4 receptor and modulates their downregulation"
**作者**: Frosi Y, et al.
**摘要**: 通过Western blot和免疫沉淀技术(使用抗ERRFI1抗体)，揭示了ERRFI1(RALT)与EGFR/ErbB4受体结合并促进其内吞降解的分子机制，为靶向治疗提供了理论依据。
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3. **文献名称**: "ERRFI1 expression predicts clinical outcomes in triple-negative breast cancer"
**作者**: Lee HJ, et al.
**摘要**: 利用抗ERRFI1抗体对三阴性乳腺癌组织进行免疫组化染色，发现高表达ERRFI1的患者总生存期更长，表明其作为潜在生物标志物的价值，并提示其通过抑制MAPK通路抑制肿瘤转移。
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以上文献均通过ERRFI1抗体在蛋白质水平研究其功能，涵盖肿瘤抑制机制、信号通路调控及临床预后分析。如需扩展，可结合具体研究领域进一步筛选。
The ERRFI1 (ERBB Receptor Feedback Inhibitor 1) antibody is a tool used to detect and study the ERRFI1 protein, also known as MIG6 (Mitogen-Inducible Gene 6) or RALT (Receptor-associated late transducer). ERRFI1 is a cytoplasmic protein encoded by the ERRFI1 gene, located on human chromosome 1p36.12. It functions as a critical negative regulator of epidermal growth factor receptor (EGFR/ERBB1) and ERBB2 (HER2) signaling pathways. By binding to activated EGFR/ERBB receptors, ERRFI1 inhibits their kinase activity, attenuates downstream signaling (e.g., MAPK/ERK, PI3K/AKT), and promotes receptor internalization, thereby maintaining cellular homeostasis and preventing uncontrolled proliferation.
ERRFI1 is implicated in various physiological processes, including cell proliferation, differentiation, and apoptosis. Its dysregulation has been linked to cancer, where reduced ERRFI1 expression correlates with enhanced EGFR/ERBB signaling, tumor progression, and poor prognosis in cancers such as lung, breast, and glioblastoma. Additionally, ERRFI1 plays roles in tissue repair, inflammation, and metabolic regulation.
The ERRFI1 antibody is widely utilized in research to assess protein expression levels, localization, and interactions via techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP). It serves as a valuable tool for investigating ERRFI1's role in cancer biology, therapeutic resistance, and as a potential biomarker for targeted therapies. Commercial ERRFI1 antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes within the protein's functional domains.
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