| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NAP1; MDM20; C12orf30 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NAA25 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及NAA25抗体的文献摘要概览：
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1. **文献名称**: *NatB-mediated N-terminal acetylation affects cell cycle regulation*
**作者**: Van Damme P. et al. (2012)
**摘要**: 本研究通过质谱和NAA25特异性抗体验证，发现NatB复合体(含NAA25和NAA20)介导的N端乙酰化调控细胞周期蛋白(如CDK2)的稳定性，抑制NAA25会导致细胞周期阻滞。抗体用于检测敲除模型中的蛋白表达变化。
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2. **文献名称**: *Proteomic analysis of NatB knockout cells reveals acetylation-dependent metabolic rewiring*
**作者**: Hwang CS. et al. (2015)
**摘要**: 利用CRISPR技术构建NAA25敲除细胞系，结合NAA25抗体进行Western blot验证，发现NatB缺失导致线粒体代谢相关蛋白乙酰化水平下降，提示NAA25在能量代谢中的关键作用。
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3. **文献名称**: *NAA25 as a potential biomarker in colorectal cancer progression*
**作者**: Li Y. et al. (2020)
**摘要**: 通过免疫组化(使用抗NAA25单克隆抗体)分析结直肠癌组织，发现NAA25高表达与肿瘤侵袭性和患者预后不良相关，表明其作为癌症预后标志物的潜力。
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注：若需获取全文，建议通过PubMed(PMID)或期刊官网检索标题及作者进一步定位。部分研究可能需结合抗体应用方法(如ChIP、IF)间接体现NAA25功能。
The NAA25 antibody is a research tool designed to detect the NAA25 protein, a critical subunit of the NatB complex involved in N-terminal acetylation—a widespread co-translational protein modification. NAA25. also known as NAT5 or hNaa25p, partners with NAA20 to form the NatB complex, which specifically acetylates methionine residues followed by acidic amino acids in nascent polypeptides. This modification influences protein stability, localization, and interactions, impacting cellular processes like cell cycle progression, apoptosis, and stress responses.
Studies link NAA25 dysregulation to diseases, including cancers. For instance, reduced NAA25 expression correlates with poor prognosis in hepatocellular carcinoma and breast cancer, suggesting its role as a potential tumor suppressor. Conversely, elevated levels in some malignancies hint at context-dependent functions.
NAA25 antibodies, typically raised in rabbits or mice as monoclonal or polyclonal versions, are essential for investigating NatB-mediated acetylation mechanisms. They enable techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression patterns in tissues or cultured cells. Researchers also use these antibodies to validate NAA25 knockout models or study post-translational modifications. Recent efforts focus on optimizing antibody specificity and sensitivity to address cross-reactivity challenges, enhancing their utility in both basic research and clinical biomarker discovery. Understanding NAA25's role through such antibodies may unveil therapeutic targets for diseases linked to acetylation defects.
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