| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HEM2; NAP1; NAP125; p125Nap1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NCKAP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NCKAP1抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*NCKAP1 regulates cell migration and invasion in glioblastoma via WAVE2 complex*
**作者**：Zhang Y, et al.
**摘要**：本研究利用NCKAP1特异性抗体，通过免疫印迹和免疫荧光技术，揭示了NCKAP1通过调控WAVE2复合体的活性，促进胶质母细胞瘤细胞的迁移和侵袭，提示其作为潜在治疗靶点。
2. **文献名称**：*A novel role of NCKAP1 in T-cell receptor signaling and immune response*
**作者**：Tanaka K, et al.
**摘要**：通过流式细胞术和共聚焦显微镜结合NCKAP1抗体，作者发现NCKAP1参与T细胞受体信号传导，影响免疫突触形成及细胞因子的分泌，为免疫调节机制提供新见解。
3. **文献名称**：*Development of a high-affinity monoclonal antibody against NCKAP1 for diagnostic applications*
**作者**：Wang L, et al.
**摘要**：本文报道了一种新型NCKAP1单克隆抗体的开发与验证，该抗体在ELISA和免疫组化中表现出高特异性，可用于癌症组织样本中NCKAP1蛋白表达的检测及预后评估。
(注：以上文献为示例，实际引用时需根据具体研究补充真实发表信息。)
**Background of NCKAP1 Antibody**
NCKAP1 (NCK-associated protein 1), also known as Hem-1. is a scaffolding protein involved in regulating cytoskeletal reorganization and cell migration, particularly in immune cells. It is a key component of the WAVE regulatory complex (WRC), which activates the Arp2/3 complex to drive actin polymerization—a critical process for cellular motility, adhesion, and immune synapse formation. NCKAP1 interacts with signaling molecules like NCK and WAVE2. linking receptor activation to cytoskeletal dynamics.
Studies highlight its role in immune function, including neutrophil chemotaxis, T-cell activation, and B-cell trafficking. Dysregulation of NCKAP1 is associated with immunodeficiencies, neurological disorders, and cancers. For example, loss-of-function mutations may impair immune cell migration, while overexpression has been observed in certain leukemias and lymphomas.
NCKAP1 antibodies are vital tools for investigating its expression, localization, and interactions in disease models. They enable techniques like Western blotting, immunoprecipitation, and immunofluorescence, aiding research on mechanisms underlying cancer metastasis, immune dysregulation, and developmental defects. These antibodies also help explore NCKAP1’s potential as a biomarker or therapeutic target in pathologies linked to cytoskeletal or signaling abnormalities.
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