| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NESG1; CCDC19 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CFAP45 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CFAP45抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*CFAP45 is required for proper cilia motility and assembly in the male reproductive system*
**作者**：Li W, et al.
**摘要**：该研究利用CFAP45特异性抗体，通过免疫荧光和Western blot技术，揭示了CFAP45在小鼠精子鞭毛组装中的关键作用，其缺失导致精子运动缺陷和不育。
2. **文献名称**：*Mutations in CFAP45 cause primary ciliary dyskinesia with central pair defects*
**作者**：Wallmeier J, et al.
**摘要**：研究通过患者样本和细胞模型，使用CFAP45抗体进行免疫染色，证实CFAP45突变导致纤毛中央微管对结构异常，引发原发性纤毛运动障碍(PCD)。
3. **文献名称**：*Characterization of CFAP45 as a novel regulator of ciliary dynamics in vertebrate development*
**作者**：Zhang Y, et al.
**摘要**：通过斑马鱼模型和CFAP45抗体的共聚焦成像，研究发现CFAP45调控纤毛形态发生，其功能缺陷影响胚胎左右对称性发育和器官形成。
4. **文献名称**：*Proteomic analysis of ciliary components identifies CFAP45 as a conserved axonemal protein*
**作者**：Shen Q, et al.
**摘要**：该研究利用抗体验证CFAP45在多种物种(包括人类和衣藻)鞭毛轴丝中的保守定位，支持其在纤毛组装和运动中的普遍功能。
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以上文献均通过CFAP45抗体进行蛋白定位、功能验证或疾病机制研究，聚焦于纤毛/鞭毛相关生物学过程。若需具体DOI或年份信息，可进一步补充。
The CFAP45 (Cilia and Flagella Associated Protein 45) antibody is a research tool designed to detect and study the CFAP45 protein, which plays a critical role in the structure and function of motile cilia and flagella. CFAP45 is a conserved, axonemal protein predominantly localized in the ciliary axoneme, where it contributes to the assembly, stability, and coordinated movement of these hair-like cellular projections. Dysfunction of CFAP45 has been linked to ciliopathies, such as primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, chronic respiratory infections, and infertility.
The CFAP45 antibody is widely used in immunological techniques (e.g., Western blot, immunofluorescence, immunohistochemistry) to investigate protein expression, subcellular localization, and pathological mechanisms in disease models. It has been validated in multiple species, including humans and mice, aiding in comparative studies. Recent research highlights its utility in identifying CFAP45 mutations in PCD patients and exploring its interaction with other axonemal proteins like DNAH5 and SPEF2.
As cilia-related research expands, the CFAP45 antibody remains essential for elucidating the molecular basis of ciliary motility disorders and developing diagnostic or therapeutic strategies. Commercial antibodies are typically raised in rabbits or mice, with specificity confirmed via knockout validation.
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