| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NEFA; HEL-S-109 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NUCB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NUCB2抗体的3篇代表性文献示例(注：以下内容为示例，实际文献需根据具体数据库检索)：
1. **文献名称**：*NUCB2/nesfatin-1 antibodies for immunohistochemical localization in rat brain tissue*
**作者**：Oh-I S, Shimada M, et al.
**摘要**：本研究开发了一种高特异性的多克隆抗体，用于检测大鼠脑组织中的NUCB2/nesfatin-1蛋白。通过免疫组化和Western blot验证，证实该抗体可特异性标记下丘脑等区域的神经元，为研究其在摄食调节中的功能提供了工具。
2. **文献名称**：*Development of a novel ELISA for quantifying human plasma NUCB2 levels in metabolic disorders*
**作者**：Zhang Y, Wang L, et al.
**摘要**：作者建立了一种基于NUCB2抗体的ELISA检测方法，用于定量分析2型糖尿病患者血浆中的NUCB2水平。研究发现，患者血浆NUCB2浓度与胰岛素抵抗呈显著正相关，提示其作为代谢疾病生物标志物的潜力。
3. **文献名称**：*NUCB2 overexpression in breast cancer: Validation by a monoclonal antibody-based assay*
**作者**：Chen X, Li R, et al.
**摘要**：通过单克隆抗体免疫组织化学分析，发现NUCB2在乳腺癌组织中高表达，并与肿瘤侵袭性和预后不良相关。该研究为NUCB2在癌症中的功能研究和临床诊断提供了抗体工具及病理学证据。
(注：以上内容为模拟示例，实际文献需以PubMed、Web of Science等平台检索为准。)
The NUCB2 antibody is designed to target nucleobindin-2 (NUCB2), a multifunctional protein encoded by the *NUCB2* gene. NUCB2 is widely expressed in various tissues, including the hypothalamus, adipose tissue, and gastrointestinal tract, and is best known as the precursor of nesfatin-1. a peptide hormone implicated in appetite regulation, energy homeostasis, and glucose metabolism. Nesfatin-1 derives from post-translational processing of NUCB2 and interacts with central and peripheral pathways to suppress food intake, modulate insulin secretion, and influence stress responses. Dysregulation of NUCB2/nesfatin-1 has been linked to metabolic disorders such as obesity, diabetes, and anorexia nervosa.
Antibodies against NUCB2 are essential tools for investigating its expression, localization, and physiological roles. They are commonly used in techniques like Western blotting, immunohistochemistry, and ELISA to quantify protein levels, assess tissue distribution, or study interactions with other molecules. Both polyclonal and monoclonal NUCB2 antibodies are available, with specificity validated via knockdown controls or knockout models. Challenges in antibody development include ensuring selectivity due to potential cross-reactivity with homologous proteins or splice variants.
Research applications span neuroscience, endocrinology, and metabolic disease studies, aiming to clarify NUCB2’s role in feeding behavior, energy balance, and its potential as a therapeutic target. Reliable NUCB2 antibodies thus underpin advancements in understanding metabolic and neuroendocrine pathways.
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