| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | NFH |
| Entrez GeneID | 4744 |
| clone | 8H10 |
| WB Predicted band size | 112.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NEFH (AA: 968-1020) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PDE5A抗体的3篇参考文献示例(注：文献信息为模拟，实际引用需核实)：
1. **"Characterization of PDE5A antibody specificity in human tissues"**
*作者：Smith J, et al. (2015)*
**摘要**：开发并验证了一种高特异性PDE5A多克隆抗体，通过免疫组化和Western blot分析，证实其在心血管和平滑肌组织中的选择性表达，为研究PDE5A的生理功能提供工具。
2. **"PDE5A expression in prostate cancer: a novel biomarker study"**
*作者：Lee C, et al. (2018)*
**摘要**：利用PDE5A单克隆抗体检测前列腺癌组织中PDE5A蛋白水平，发现其过表达与肿瘤侵袭性相关，提示其作为潜在治疗靶点的可能性。
3. **"Localization of PDE5A in pulmonary hypertension using immunofluorescence"**
*作者：Garcia R, et al. (2020)*
**摘要**：通过免疫荧光技术结合PDE5A抗体，揭示肺高压患者肺血管中PDE5A的空间分布变化，为靶向药物疗效评估提供形态学依据。
**建议**：实际研究中请通过PubMed或Google Scholar搜索关键词“PDE5A antibody + application/expression”获取最新文献，并核实准确性。
The PDE5A antibody is a specialized tool used to detect and study phosphodiesterase type 5A (PDE5A), an enzyme critical in regulating cyclic guanosine monophosphate (cGMP) signaling. PDE5A hydrolyzes cGMP, a secondary messenger involved in smooth muscle relaxation, vasodilation, and neurotransmission. It is notably expressed in vascular smooth muscle, platelets, and the corpus cavernosum, making it a therapeutic target for conditions like erectile dysfunction (e.g., sildenafil/Viagra) and pulmonary arterial hypertension.
PDE5A antibodies are essential in research to investigate the enzyme's expression, localization, and function in physiological and pathological contexts. They are utilized in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to quantify PDE5A levels in tissues or cell lines. These antibodies also aid in studying PDE5A's role in cardiovascular diseases, cancer (where PDE5A overexpression is linked to tumor progression), and neurological disorders.
Most PDE5A antibodies are raised against specific epitopes of human PDE5A, often targeting conserved regions across species for cross-reactivity. Validation includes knockout controls or enzymatic activity assays to ensure specificity. Researchers prioritize monoclonal antibodies for consistency or polyclonal versions for detecting multiple isoforms (PDE5A1. A2. A3). Reliable PDE5A antibodies are vital for advancing studies on cGMP pathway dysregulation and developing targeted therapies.
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