首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人雌激素(E)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的E含量。试剂盒检测范围为25 pg/mL-1000 pg/mL，该产品可广泛应用于基础医学研究中激素动态监测、生殖生物学机制探索、环境内分泌干扰物评估，以及药物开发过程中雌激素相关代谢通路的体外分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：E
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：25 pg/mL-1000 pg/mL
灵敏度：25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 86-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 94-101 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of human estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 02.3912.3512.371 251.5421.5391.541 750.8650.8420.854 2300.5670.5650.566 5000.3610.3590.360 10000.1560.1530.155 | ||||||
货期：3-5 working days
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