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产品描述：人表达于SiSo细胞系的受体结合型肿瘤抗原（RCAS1）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆样本中的EBAG9含量。EBAG9是一种雌激素受体结合位点相关抗原，被鉴定为雌激素反应基因。其研究机制涉及EBAG9如何抑制T细胞反应，影响抗肿瘤免疫响应，以及其在肿瘤免疫逃避和肿瘤恶化中的作用。试剂盒检测范围为0.78 U/mL-50 U/mL，本产品可为探索肿瘤微环境调控机制及生物标志物筛选提供技术支持。适用于体外定量分析血清样本中循环RCAS1水平或肿瘤组织局部表达量，为癌症发生发展机制研究、药物靶点验证及实验动物模型评估等科研领域提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BAG9 ELISA Kit; Cancer associated surface antigen ELISA Kit; Cancer associated surface antigen RCAS1 ELISA Kit; Cancer-associated surface antigen RCAS1 ELISA Kit; EB9 ELISA Kit; EBAG 9 ELISA Kit; EBAG9 ELISA Kit; Estrogen receptor binding fragment associated gene 9 protein ELISA Kit; Estrogen receptor binding site associated antigen 9 ELISA Kit; estrogen receptor binding site associated; antigen; 9 ELISA Kit; Estrogen receptor-binding fragment-associated gene 9 protein ELISA Kit; PDAF ELISA Kit; RCAS 1 ELISA Kit; RCAS1 ELISA Kit; RCAS1_HUMAN ELISA Kit; Receptor binding cancer antigen expressed on SiSo cells ELISA Kit; Receptor-binding cancer antigen expressed on SiSo cells ELISA Kit
缩写：EBAG9
Uniprot No.：O00559
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates
检测范围：0.78 U/mL-50 U/mL
灵敏度：0.195 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RCAS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 99-111 | |||||
回收率：
| The recovery of human RCAS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| U/mlOD1OD2AverageCorrected502.7022.7582.7302.577252.2112.2192.2152.06212.51.5661.5961.5811.4286.250.9840.9970.9910.8383.120.5620.5810.5720.4191.560.4390.4070.4230.2700.780.2910.2810.2860.13300.1510.1540.153 | ||||||
货期：3-5 working days
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