| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | SIR2L4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SIRT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与SIRT4抗体相关的代表性文献摘要信息：
1. **文献名称**：SIRT4 Inhibits Fatty Acid Oxidation and Mitochondrial Gene Expression in Hepatocytes
**作者**：Mathias RA et al.
**摘要**：该研究利用SIRT4特异性抗体验证其在肝脏细胞中的定位，证明SIRT4通过ADP-核糖基化抑制脂肪酸氧化关键酶，调控线粒体代谢通路。(Cell Metabolism, 2014)
2. **文献名称**：SIRT4 Represses Peroxisome Proliferator-Activated Receptor Activity via Deacetylation
**作者**：Laurent G et al.
**摘要**：研究采用SIRT4抗体进行免疫共沉淀实验，揭示SIRT4通过去乙酰化修饰抑制PPARα转录活性，影响脂质代谢稳态。(Genes & Development, 2013)
3. **文献名称**：SIRT4 Suppresses Tumorigenesis by Coordinating Mitochondrial One-Carbon Metabolism
**作者**：Nakamura Y et al.
**摘要**：通过Western Blot和免疫组化(使用SIRT4抗体)，发现SIRT4缺失导致线粒体一碳代谢紊乱，促进肿瘤发生。(Molecular Cell, 2020)
注：以上文献需通过PubMed/Web of Science等平台搜索标题获取全文，实验方法部分包含抗体应用细节。如需更多文献可进一步补充。
SIRT4. a member of the sirtuin family of NAD⁺-dependent deacylases, is a mitochondrial enzyme involved in metabolic regulation, including insulin secretion, fatty acid oxidation, and amino acid metabolism. Unlike other sirtuins (e.g., SIRT1 or SIRT3), SIRT4 primarily exhibits ADP-ribosyltransferase activity, though weak deacetylase activity has also been reported. It plays a tumor-suppressive role by modulating energy homeostasis and inhibiting glutamine metabolism, linking it to cancer biology and metabolic disorders.
SIRT4 antibodies are critical tools for studying its expression, localization, and post-translational modifications. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect endogenous SIRT4 in tissues or cell lines. Given SIRT4’s low abundance and tissue-specific expression, high specificity and sensitivity are essential for reliable detection. Validated antibodies often undergo testing in SIRT4-knockout models or siRNA-treated cells to confirm target specificity.
Research applications include exploring SIRT4’s role in aging, mitochondrial dysfunction, diabetes, and cancer. Commercially available antibodies vary in host species (e.g., rabbit, mouse), epitope regions, and cross-reactivity (human, mouse, rat). Challenges include distinguishing SIRT4 from other sirtuins due to structural homology and ensuring consistent performance across experimental conditions. Selecting a well-validated antibody is crucial for accurate data interpretation in studies targeting metabolic pathways or therapeutic development.
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