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产品描述：人白介素27β亚基(EBI3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EBI3含量。EBI3（爱泼斯坦-巴尔病毒诱导的3）是一种编码分泌型糖蛋白的基因，通过B淋巴细胞响应EB病毒感染而表达。它异源二聚化与28 kDa的蛋白质形成IL-27，调节T细胞和炎症反应，部分通过激活CD4+ T细胞的JAK/STAT通路。EBI3在免疫调节和炎症反应中起关键作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，支持科研人员开展免疫调控机制研究、炎症相关疾病模型分析，以及通过检测生物样本中EBI3的表达动态探索其作为潜在生物标志物的可能性，为免疫学、分子病理学等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cytokine receptor ELISA Kit; EBI3 ELISA Kit; EBV induced gene 3 protein ELISA Kit; EBV-induced gene 3 protein ELISA Kit; Epstein Barr virus induced 3 ELISA Kit; Epstein Barr virus induced gene 3 ELISA Kit; Epstein Barr virus induced gene 3 protein ELISA Kit; Epstein-Barr virus-induced gene 3 protein ELISA Kit; IL 27 subunit beta ELISA Kit; IL 27B ELISA Kit; IL-27 subunit beta ELISA Kit; IL-27B ELISA Kit; IL27 subunit ELISA Kit; IL27 subunit beta ELISA Kit; IL27B ELISA Kit; IL27B_HUMAN ELISA Kit; IL35 subunit ELISA Kit; interleukin 27 subunit beta ELISA Kit; Interleukin-27 subunit beta ELISA Kit
缩写：EBI3
Uniprot No.：Q14213
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EBI3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-105 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human EBI3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-92 | ||||
| EDTA plasma (n=4) | 101 | 96-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6432.5892.6162.4765001.6781.6451.6621.5222501.0561.0331.0450.9051250.7020.7140.7080.56862.50.4520.4760.4640.32431.250.2970.3080.3030.16315.60.2150.2220.2190.07900.1390.1410.140 | ||||||
货期：3-5 working days
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