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产品描述：人细胞外基质蛋白1(ECM1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ECM1含量。ECM1是一种分泌型糖蛋白，在软骨内骨形成、血管生成和肿瘤生物学中发挥关键作用。研究发现，ECM1在多种肿瘤中高表达，可能与血管生成、肿瘤进展和恶性转化有关。研究机制显示，ECM1通过调节细胞外基质结构、促进细胞增殖和迁移等途径影响肿瘤生物学行为。试剂盒检测范围为78 pg/mL-5000 pg/mL，为研究银屑病、系统性硬化症等皮肤相关疾病、肿瘤转移机制及器官纤维化进程提供高效工具，可满足体外实验中对 ECM1 动态变化的定量分析需求，为探索其在细胞外基质调控、病理生理机制中的功能提供可靠支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ECM 1 ELISA Kit; Ecm1 ELISA Kit; ECM1_HUMAN ELISA Kit; Extracellular matrix protein 1 ELISA Kit; Secretory component p85 ELISA Kit; URBWD ELISA Kit
缩写：ECM1
Uniprot No.：Q16610
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ECM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 84-95 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of human ECM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 86 | 81-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.4382.5322.4852.35925001.4281.5271.4781.35212500.8360.8560.8460.7206250.5130.5270.5200.3943120.3080.3150.3120.1861560.2450.2570.2510.125780.2040.2080.2060.08000.1250.1270.126 | ||||||
货期：3-5 working days
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