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产品描述：人包含EGF腓骨蛋白样胞外基质蛋白1(EFEMP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EFEMP1含量。EFEMP1是一种与细胞代谢密切相关的重要细胞外基质蛋白，属于fibulin家族成员。研究发现，EFEMP1在多种癌症中表达异常，可抑制或促进肿瘤细胞的侵袭转移。其作用机制可能与抑制MMP-7表达、调控细胞信号通路、影响肿瘤微环境等因素有关。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于探究EFEMP1在组织纤维化、肿瘤微环境调控或血管生成等领域的分子机制研究，也可用于疾病模型中蛋白动态变化的纵向监测。支持科研人员开展体外样本的定量分析，为探索EFEMP1的生物学功能及其在疾病发生发展中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DHRD ELISA Kit; Doyne honeycomb retinal degeneration ELISA Kit; DRAD ELISA Kit; EFEMP 1 ELISA Kit; EFEMP1 ELISA Kit; EGF containing fibulin like extracellular matrix protein 1 ELISA Kit; EGF containing fibulin like extracellular matrix protein 1 precursor ELISA Kit; EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Epidermal growth factor containing fibulin like extracellular matrix protein 1 ELISA Kit; Extracellular protein S1 5 ELISA Kit; Extracellular protein S1-5 ELISA Kit; FBLN 3 ELISA Kit; FBLN3 ELISA Kit; FBLN3_HUMAN ELISA Kit; FBNL ELISA Kit; FIBL 3 ELISA Kit; FIBL-3 ELISA Kit; FIBL3 ELISA Kit; Fibrillin like ELISA Kit; Fibrillin like protein ELISA Kit; Fibrillin-like protein ELISA Kit; FIBULIN 3 ELISA Kit; Fibulin-3 ELISA Kit; Fibulin3 ELISA Kit; FLJ35535 ELISA Kit; Heat shock 70 KD protein 1 ELISA Kit; MGC111353 ELISA Kit; MLVT ELISA Kit; MTLV ELISA Kit; S1 5 ELISA Kit; T16 protein ELISA Kit
缩写：EFEMP1
Uniprot No.：Q12805
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 98-109 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of human EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-94 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.2612.1272.1942.074501.5391.5001.5201.400250.8540.9020.8780.75812.50.5170.5450.5310.4116.250.3150.3040.3100.1903.120.2260.2330.2300.1101.560.1700.1820.1760.05600.1210.1180.120 | ||||||
货期：3-5 working days
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