首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人内皮素2(EDN2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EDN2含量。试剂盒检测范围为4.688 pg/mL-300 pg/mL，该产品适用于基础科研领域，可用于研究EDN2在生殖生理、心血管调节或肿瘤微环境中的表达规律，为探索其分子机制、构建疾病模型或评估药物干预效果提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EDN2 ELISA Kit; EDN2_HUMAN ELISA Kit; Endothelin 2 ELISA Kit; Endothelin-2 ELISA Kit; ET 2 ELISA Kit; ET-2 ELISA Kit; ET2 ELISA Kit; PPET2 ELISA Kit; Preproendothelin 2 ELISA Kit; Preproendothelin-2 ELISA Kit
缩写：EDN2
Uniprot No.：P20800
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：4.688 pg/mL-300 pg/mL
灵敏度：1.172 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 103 | |||||
| Range % | 96-107 | ||||||
| 1:2 | Average % | 86 | |||||
| Range % | 81-92 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 86 | |||||
| Range % | 80-91 | ||||||
回收率：
| The recovery of human EDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 87-98 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected3001.8321.9881.9101.8021501.2141.1921.2031.095750.7600.7210.7410.63337.50.4420.4510.4470.33918.750.2870.2760.2820.1749.3750.2120.2170.2150.1074.6880.1550.1600.1580.05000.1070.1080.108 | |||||||
货期：3-5 working days
×
