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产品描述:人EGF样重复序列和盘状结构域蛋白3(EDIL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的EDIL3含量。EDIL3,全称EGF-like repeats and discoidin I-like domains 3,是一种细胞外基质蛋白,在胚胎发育过程中由内皮细胞分泌,并在多种肿瘤细胞中表达。它通过其RGD模体结合整合素αvβ3,促进血管生成和抗炎作用,同时其盘状结构域可识别磷脂酰丝氨酸,协助吞噬凋亡细胞和清除血小板微粒。在肿瘤微环境中,EDIL3的异常表达可能与肿瘤生长、血管生成和转移有关。试剂盒检测范围为125 pg/mL-8000 pg/mL,该产品适用于基础科研领域,如探究 EDIL3 在肿瘤微环境、心血管疾病模型或炎症相关疾病中的表达调控机制,也可用于体外药物干预或基因编辑后靶蛋白水平的动态评估,为分子机制研究提供可靠工具。试剂盒操作便捷,配套标准品及预包被板条,兼容常规实验室检测流程,适用于细胞生物学、病理学等领域的定量分析需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:DEL1 ELISA Kit; Developmental endothelial locus 1 ELISA Kit; Developmentally regulated endothelial cell locus 1 protein ELISA Kit; Developmentally-regulated endothelial cell locus 1 protein ELISA Kit; Edil3 ELISA Kit; EDIL3_HUMAN ELISA Kit; EGF like repeat and discoidin I like domain containing protein 3 ELISA Kit; EGF like repeats and discoidin I like domains 3 ELISA Kit; EGF like repeats and discoidin I like domains protein 3 ELISA Kit; EGF-like repeat and discoidin I-like domain-containing protein 3 ELISA Kit; Integrin binding protein DEL1 ELISA Kit; Integrin-binding protein DEL1 ELISA Kit
缩写:EDIL3
Uniprot No.:O43854
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:125 pg/mL-8000 pg/mL
灵敏度:31.25 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human EDIL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 87 | ||||
Range % | 80-95 | |||||
1:2 | Average % | 95 | ||||
Range % | 89-100 | |||||
1:4 | Average % | 99 | ||||
Range % | 91-110 | |||||
1:8 | Average % | 94 | ||||
Range % | 88-98 |
回收率:
The recovery of human EDIL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 80-98 | ||||
EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected80002.0331.8721.9531.76540001.5981.4761.5371.34920001.0771.0141.0460.85810000.7740.7290.7520.5645000.5130.4940.5040.3162500.3740.3590.3670.1791250.2910.2780.2850.09700.1900.1860.188 |
货期:3-5 working days
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