首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：人肿瘤坏死因子受体超家族成员(EDA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EDA含量。人肿瘤坏死因子受体超家族成员EDA，是一种非死亡受体，主要在神经系统和毛囊中发挥生理功能。其在癌症中具有促癌和抑癌的双重作用，如促进胶质瘤和鼻咽癌的发展，同时在胃癌和肺癌中发挥抑癌作用。EDA通过激活NF-κB和JNK信号通路，参与细胞凋亡、增殖和分化等生物学过程。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于生命科学研究领域，包括免疫调节机制探索、疾病模型建立、药物开发过程中的生物标志物检测等应用场景，特别适合需要高灵敏度和宽检测范围的体外实验需求，可满足细胞实验、动物模型研究及基础医学研究中的蛋白表达分析需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Eda; Ed1; Ta; Ectodysplasin-A; EDA protein homolog; Tabby protein
缩写：EDA
Uniprot No.：O54693
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse EDA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-107 | |||||
回收率：
| The recovery of mouse EDA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.3832.2532.3182.161 5001.7821.7531.7681.611 2501.0971.1451.1210.964 1250.6930.7400.7170.560 62.50.4500.4610.4560.299 31.250.3160.3050.3110.154 15.60.2510.2420.2470.090 00.1590.1540.157 | ||||||
货期：3-5 working days
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