首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人嗜酸性粒细胞阳离子蛋白(ECP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ECP含量。ECP（体外光化学疗法）是一种免疫调节疗法，通过收集人体外周血液中的免疫细胞，暴露于光敏剂和紫外线照射后重新输注回体内，用于治疗急性移植物抗宿主病。其作用机制尚不完全清楚，目前的研究主要基于回顾性或观察性研究，建议在随机对照试验（RCTs）背景下使用。试剂盒检测范围为1.56 ng/mL-100 ng/mL，本试剂盒适用于基础医学研究、疾病机制探索及药物开发等科研领域，支持多种样本类型检测：血清和血浆样本可用于分析体内ECP动态水平，而组织匀浆检测则适用于动物模型或体外实验中局部组织炎症反应的评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cytotoxic ribonuclease ELISA Kit; ECP ELISA Kit; ECP_HUMAN ELISA Kit; Eosinophil cationic protein ELISA Kit; OTTHUMP00000164017 ELISA Kit; Ribonuclease 3 ELISA Kit; Ribonuclease; RNase A family; 3 ELISA Kit; RNase 3 ELISA Kit; RNASE3 ELISA Kit; RNS3 ELISA Kit
缩写：ECP
Uniprot No.：P12724
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 94-108 | |||||
| 1:8 | Average % | 108 | ||||
| Range % | 103-112 | |||||
回收率：
| The recovery of human ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 92 | 89-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.5002.4932.4972.392502.0031.9982.0011.896251.2751.2661.2711.16612.50.5870.5730.5800.4756.250.3690.3570.3630.2583.120.2210.2080.2150.1101.560.1760.1690.1730.06800.1080.1020.105 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ECP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ECP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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