首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠EGF样重复序列和盘状结构域蛋白3(EDIL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的EDIL3含量。试剂盒检测范围为28 pg/mL-1800 pg/mL，研究人员可通过本产品探究EDIL3在肿瘤微环境调控、血管内皮细胞功能或再生医学研究中的动态变化，例如评估疾病模型动物特定组织中EDIL3的表达模式，或体外培养细胞分泌EDIL3的调控机制，为相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Edil3 ELISA kit; Del1EGF-like repeat and discoidin I-like domain-containing protein 3 ELISA kit; Developmentally-regulated endothelial cell locus 1 protein ELISA kit; Integrin-binding protein DEL1 ELISA kit
缩写：EDIL3
Uniprot No.：O35474
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse EDIL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-100 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 88-95 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of mouse EDIL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 98 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18002.8122.9982.9052.7439002.2152.3452.2802.1184501.8031.8041.8041.6422251.2101.2611.2361.074112.50.6720.7200.6960.534560.4060.4310.4190.257280.2700.2510.2610.09900.1570.1660.162 | ||||||
货期：3-5 working days
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