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产品描述：人肝配蛋白A1受体(EFNA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EFNA1含量。EFNA1 即 EphrinA1，是 Eph 受体酪氨酸激酶的配体。它参与多种生理病理过程，如血管生成、细胞迁移等。研究机制方面，其通过与 Eph 受体相互作用，激活下游信号通路，影响细胞行为，在肿瘤、心血管疾病等研究中受关注。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本产品为科研领域研究EFNA1在肿瘤侵袭转移、血管异常增生或细胞迁移调控中的分子机制提供可靠工具，支持体外实验中对不同来源样本的蛋白表达水平分析，广泛应用于癌症生物学、细胞信号转导及发育相关的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B61 ELISA Kit; ECKLG ELISA Kit; EFL 1 ELISA Kit; EFL1 ELISA Kit; EFNA 1 ELISA Kit; Efna1 ELISA Kit; EFNA1_HUMAN ELISA Kit; EPH related receptor tyrosine kinase ligand 1 ELISA Kit; EPH-related receptor tyrosine kinase ligand 1 ELISA Kit; Ephrin-A1 ELISA Kit; Ephrin-A1, secreted form ELISA Kit; EphrinA1 ELISA Kit; EPLG 1 ELISA Kit; EPLG1 ELISA Kit; Immediate early response protein B61 ELISA Kit; LERK 1 ELISA Kit; LERK-1 ELISA Kit; LERK1 ELISA Kit; Ligand of eph related kinase 1 ELISA Kit; OTTHUMP00000033242 ELISA Kit; OTTHUMP00000033271 ELISA Kit; secreted form ELISA Kit; TNF alpha-induced protein 4 ELISA Kit; TNFAIP 4 ELISA Kit; TNFAIP4 ELISA Kit; Tumor necrosis factor alpha induced protein 4 ELISA Kit; Tumor necrosis factor alpha-induced protein 4 ELISA Kit
缩写：EFNA1
Uniprot No.：P20827
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human EFNA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of Human EFNA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 86 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.9182.7172.8182.70152.4252.5242.4752.3582.51.9131.8161.8651.7481.251.1301.1821.1561.0390.6250.5800.5470.5640.4470.3120.3450.3310.3380.2210.1560.2070.2110.2090.09200.1160.1170.117 | ||||||
货期：3-5 working days
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