首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠含EGF纤维蛋白细胞外基质蛋白 1(EFEMP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EFEMP1含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品可为研究EFEMP1在小鼠模型中的生物学功能提供可靠工具，例如探索其在组织修复、肿瘤转移或眼部疾病中的表达动态，亦可用于基因编辑动物模型的表型验证及药物干预实验的效果评估，满足基础科研中对EFEMP1蛋白水平的精准定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Efemp1 ELISA Kit; Fbln3EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Fibulin-3 ELISA Kit; FIBL-3 ELISA Kit
缩写：EFEMP1
Uniprot No.：Q8BPB5
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:2 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
回收率：
| The recovery of mouse EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 85-94 | |||||
| EDTA plasma (n=4) | 97 | 93-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.2432.1682.2062.03310001.5531.6051.5791.4065001.0161.0131.0150.8422500.5970.5740.5860.4131250.3880.4120.4000.22762.50.3240.3370.3310.15831.250.2770.2710.2740.10100.1770.1690.173 | |||||||
货期：3-5 working days
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