| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NCX3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human SLC8A3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下为3篇关于SLC8A3抗体的参考文献示例(内容基于虚构假设，实际文献需通过学术数据库检索)：
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1. **文献名称**: "SLC8A3 Antibody Validation in Murine Cardiac Tissue"
**作者**: Zhang et al. (2018)
**摘要**: 该研究验证了一种新型SLC8A3特异性抗体在小鼠心肌细胞中的适用性，通过Western blot和免疫荧光确认其特异性，并发现SLC8A3在心肌缺血后表达上调，提示其在钙稳态中的潜在作用。
2. **文献名称**: "Role of NCX3 (SLC8A3) in Neuronal Calcium Homeostasis: Antibody-Based Localization Studies"
**作者**: Rossi et al. (2015)
**摘要**: 利用SLC8A3抗体进行大鼠脑组织免疫组化分析，揭示NCX3蛋白在海马体和皮层神经元中的高表达，并证明其在神经兴奋性损伤后钙外排中的保护功能。
3. **文献名称**: "SLC8A3 Knockout Model Characterization Using CRISPR and Antibody-Based Phenotyping"
**作者**: Kim & Lee (2020)
**摘要**: 通过CRISPR技术构建SLC8A3基因敲除小鼠，结合特异性抗体验证蛋白缺失，发现该基因缺失导致骨骼肌收缩功能异常，支持SLC8A3在肌肉钙调控中的必要性。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词如“SLC8A3 antibody validation”或“NCX3 antibody application”。
The solute carrier family 8 member 3 (SLC8A3), also known as the sodium-calcium exchanger 3 (NCX3), is a transmembrane protein encoded by the SLC8A3 gene. It plays a critical role in calcium homeostasis by mediating the electrogenic exchange of Na⁺ and Ca²⁺ across cellular membranes. NCX3 is predominantly expressed in the brain, skeletal muscle, and mitochondria, where it regulates intracellular calcium levels essential for neuronal signaling, muscle contraction, and mitochondrial function. Dysregulation of NCX3 has been implicated in neurodegenerative disorders, ischemic brain injury, and muscle pathologies.
SLC8A3 antibodies are laboratory tools designed to detect and quantify the NCX3 protein in various experimental applications, including Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are typically produced using immunogenic peptides derived from conserved regions of the NCX3 protein, ensuring specificity across human, mouse, and rat samples. Validation often involves knockout cell lines or tissues to confirm target absence.
Research utilizing SLC8A3 antibodies has advanced understanding of calcium signaling in neurological and muscular systems. For instance, studies highlight NCX3's neuroprotective role during cerebral ischemia and its involvement in mitochondrial calcium buffering. Commercially available antibodies vary in clonality (monoclonal/polyclonal) and conjugate formats, with selection depending on experimental needs. Reliable SLC8A3 antibodies are critical for exploring its therapeutic potential in diseases like Alzheimer's, Parkinson's, or muscular dystrophy.
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