首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肝配蛋白A1受体(EFNA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EFNA1含量。EFNA1 即艾弗林 A1，是 Eph 受体酪氨酸激酶的膜结合配体。在肿瘤等疾病中，EFNA1 与其受体结合，参与调节细胞黏附、迁移、增殖等。研究聚焦其在肿瘤血管生成、侵袭转移机制，有望为相关疾病治疗提供新靶点和策略。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，适用于基础科研领域，如探究肝损伤模型、肿瘤微环境或再生医学研究中EFNA1的表达调控机制，亦可应用于药物干预实验或信号通路研究中靶点蛋白的动态监测，为分子机制研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Efna1 ELISA Kit; Epgl1 ELISA Kit; Epl1 ELISA Kit; Lerk1 ELISA Kit; Ephrin-A1 ELISA Kit; EPH-related receptor tyrosine kinase ligand 1 ELISA Kit; LERK-1 ELISA Kit; Immediate early response protein B61) [Cleaved into: Ephrin-A1 ELISA Kit; secreted form] ELISA Kit
缩写：EFNA1
Uniprot No.：P52793
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.43 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse EFNA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 83-90 | ||||||
| 1:8 | Average % | 105 | |||||
| Range % | 100-110 | ||||||
回收率：
| The recovery of mouse EFNA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 80-94 | |||||
| EDTA plasma (n=4) | 102 | 98-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected60002.7022.6942.6982.53030002.1842.2012.1932.02515001.5851.6021.5941.4267501.0881.0691.0790.9113750.6690.6790.6740.506187.50.4530.4680.4610.29393.750.3140.3300.3220.15400.1680.1670.168 | |||||||
货期：3-5 working days
×
