首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：牛表皮生长因子(EGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的EGF含量。EGF（表皮生长因子）是EGF家族的创始成员，具有53个氨基酸残基和三个分子内二硫键。通过与细胞表面的EGFR受体结合，EGF刺激细胞生长、分化和存活，参与多种生理和病理过程，如组织再生、伤口愈合和肿瘤发生。EGF通过激活EGFR的酪氨酸激酶活性，启动信号转导级联反应，导致细胞内钙水平升高、糖酵解和蛋白质合成增加，最终促进DNA合成和细胞增殖。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品可广泛应用于动物生理学研究、营养代谢评估、疾病模型构建等科研领域，为探究牛EGF在生长发育调控、免疫应答及病理过程中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HOMG4, URG, beta-urogastrone|epidermal growth factor
缩写：EGF
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 106 | ||||
| Range % | 100-109 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-97 | |||||
回收率：
| The recovery of bovine EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 94 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.1092.0422.0761.95251.7101.7231.7171.5932.51.4321.4211.4271.3031.251.0180.9991.0090.8850.6250.6780.6680.6730.5490.3120.4660.4510.4590.3350.1560.2990.2920.2960.17200.1250.1230.124 | ||||||
货期：3-5 working days
×
