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产品描述：鸡表皮生长因子(EGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EGF含量。EGF即表皮生长因子，是一种广泛存在于人和动物体内的小分子多肽。其可促进细胞分裂增殖。在研究机制方面，它能与细胞表面的EGF受体结合，激活细胞内信号通路，在组织修复、肿瘤发生发展等研究领域备受关注。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于家禽生长调控、组织修复等基础研究领域；科研人员可通过动态监测实验动物EGF分泌水平，用于家禽生理机制研究、营养干预实验或疾病模型中的生长因子表达分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：EGF
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 100-104 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 85-91 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 96-102 | |||||
回收率：
| The recovery of chicken EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 102-106 | ||||
| EDTA plasma (n=4) | 93 | 90-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.3372.4662.4022.28940001.8931.8751.8841.77120001.4521.4551.4541.34110001.0641.0991.0820.9695000.6430.6320.6380.5252500.4430.4780.4610.3481250.2720.2950.2840.17100.1140.1120.113 | ||||||
货期：3-5 working days
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