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产品描述：人表皮生长因子样蛋白6(EGFL6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EGFL6含量。EGFL6作为靶点，其它在人体生理或病理过程中具有一定作用。研究机制上，可能涉及参与细胞间信号传导、影响细胞的增殖、迁移等活动，目前科学家正深入研究其在疾病发生发展中的具体作用机制，以探寻相关治疗策略。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于体外研究EGFL6在肿瘤发生发展、血管生成相关疾病中的表达模式，也可用于细胞培养上清或组织样本的蛋白动态监测，为探索其分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EGF like domain multiple 6 ELISA Kit; EGF repeat containing protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; EGF-like-domain multiple 6 ELISA Kit; egfl6 ELISA Kit; EGFL6_HUMAN ELISA Kit; epidermal growth factor like protein 6 ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; MAEG ELISA Kit; MAM and EGF domain containing ELISA Kit; MAM and EGF domains containing gene protein ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; W80 ELISA Kit
缩写：EGFL6
Uniprot No.：Q8IUX8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 93-105 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 89-101 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 94-109 | ||||||
回收率：
| The recovery of human EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 83-94 | |||||
| EDTA plasma (n=4) | 102 | 95-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected50001.9721.9181.9451.80025001.4001.4391.4201.27512501.0721.0251.0490.9046250.7620.7340.7480.6033120.5210.5290.5250.3801560.3480.3570.3530.208780.2710.2830.2770.13200.1480.1420.145 | |||||||
货期：3-5 working days
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