| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RRG1; RAI15; NN8-4AG; ZMYND23 |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SMYD5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SMYD5抗体的3篇参考文献示例(内容基于公开研究概括，部分为模拟虚构，实际文献请通过学术数据库查询)：
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1. **文献名称**：*SMYD5 regulates hematopoietic stem cell maintenance through histone H3 lysine 36 methylation*
**作者**：Lee, J. et al.
**摘要**：本研究揭示了SMYD5作为组蛋白甲基转移酶在造血干细胞中的关键作用，通过特异性抗体验证其在H3K36me2修饰中的活性，并证明其缺失导致干细胞分化异常。
2. **文献名称**：*SMYD5-mediated epigenetic silencing promotes tumor progression in triple-negative breast cancer*
**作者**：Zhang, Y. et al.
**摘要**：研究者利用SMYD5抗体进行染色质免疫沉淀(ChIP)，发现其通过抑制抑癌基因的启动子甲基化促进乳腺癌侵袭，为靶向治疗提供潜在靶点。
3. **文献名称**：*Development and validation of a SMYD5-specific monoclonal antibody for functional studies*
**作者**：Smith, R.K. & Patel, T.
**摘要**：本文报道了一种高特异性SMYD5单克隆抗体的开发，验证其在Western blot、免疫荧光及共沉淀实验中的可靠性，并应用于探究SMYD5与DNA损伤修复的关联。
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如需具体文献，建议通过PubMed或Google Scholar以“SMYD5 antibody”或“SMYD5 function”为关键词检索。
The SMYD5 antibody is a research tool designed to detect and study the SMYD5 protein, a member of the SMYD (SET and MYND domain-containing) family of lysine methyltransferases. SMYD5 contains a conserved SET domain responsible for methyltransferase activity and a MYND domain implicated in protein-protein interactions. Unlike other SMYD proteins (e.g., SMYD1-4), SMYD5 is less characterized but is proposed to play roles in epigenetic regulation, chromatin remodeling, and transcriptional repression. Studies suggest it may methylate histone H4 at lysine 20 (H4K20) or non-histone targets, influencing DNA repair, cell cycle progression, and immune responses. Dysregulation of SMYD5 has been linked to cancers, autoimmune disorders, and neurodegenerative diseases, though its precise mechanisms remain under investigation.
The SMYD5 antibody is typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. It is validated for applications such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specificity is confirmed via knockout controls or siRNA-mediated knockdown. Researchers use this antibody to explore SMYD5's expression patterns, subcellular localization (primarily nuclear), and interactions with chromatin-modifying complexes. Its role in diseases, particularly in oncogenesis and inflammation, makes it a focus for therapeutic targeting studies. Commercial SMYD5 antibodies vary in clonality, epitope recognition, and cross-reactivity across species (human, mouse, rat), necessitating careful selection based on experimental needs.
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