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产品描述：猪表皮生长因子(EGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EGF含量。EGF（表皮生长因子）是EGF家族的创始成员，具有与家族成员高度相似的结构和功能。EGF是一种低分子量多肽，最初从小鼠颌下腺中纯化，后在多种人体组织中发现。EGF通过与细胞表面的EGFR受体结合，刺激细胞增殖、分化和存活，在伤口愈合、组织修复等方面发挥重要作用。试剂盒检测范围为250 pg/mL-10000 pg/mL，可广泛应用于动物营养学、生殖生理学、免疫学等基础研究领域，例如探究饲料添加剂对肠道黏膜修复的影响、评估应激状态下EGF的动态变化，或解析特定疾病模型中生长因子调控机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：EGF
Uniprot No.：F1S137
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：250 pg/mL-10000 pg/mL
灵敏度：125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 97-105 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-90 | |||||
回收率：
| The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 83-89 | ||||
| EDTA plasma (n=4) | 101 | 99-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 100002.6472.6872.667 50001.8391.8991.869 25001.0571.0671.062 12500.5990.5840.592 5000.2410.2310.236 2500.1120.1140.113 | ||||||
货期：3-5 working days
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