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产品描述：人ELAVL1样蛋白1(ELAVL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ELAVL1含量。ELAVL1是一种RNA结合蛋白，在细胞生长、分化等生理过程中发挥重要作用。研究发现它通过与特定RNA结合，调控其稳定性、转运和翻译等。其异常表达与多种疾病相关，如肿瘤发生发展，目前针对它的作用机制研究是热点，有望为相关疾病治疗提供新靶点。试剂盒检测范围为25 pg/mL-1600 pg/mL，该产品可广泛应用于分子生物学研究领域，如探索ELAVL1在肿瘤细胞信号通路中的调控机制、评估特定处理条件下蛋白表达水平变化，或用于神经退行性疾病模型中RNA结合蛋白的功能研究，为科研人员提供稳定可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ELAV (embryonic lethal abnormal vision Drosophila) like 1 ELISA Kit; ELAV (embryonic lethal; abnormal vision; Drosophila) like 1 (Hu antigen R) ELISA Kit; ELAV like 1 ELISA Kit; ELAV like RNA binding protein 1 ELISA Kit; ELAV-like protein 1 ELISA Kit; ELAV1 ELISA Kit; ELAV1_HUMAN ELISA Kit; Elavl1 ELISA Kit; Embryonic lethal abnormal vision drosophila homolog like 1 ELISA Kit; Hu Antigen R ELISA Kit; Hu-antigen R ELISA Kit; Hua ELISA Kit; HuR ELISA Kit; MelG ELISA Kit
缩写：ELAVL1
Uniprot No.：Q15717
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ELAVL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 89-99 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-101 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 88-98 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of human ELAVL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 94-102 | |||||
| EDTA plasma (n=4) | 87 | 83-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.1672.0582.1131.9568001.6431.5531.5981.4414001.1661.1371.1520.9952000.7870.7590.7730.6161000.5420.5360.5390.382500.3680.3520.3600.203250.2790.2710.2750.11800.1580.1560.157 | |||||||
货期：3-5 working days
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