首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人弹性蛋白(ELN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物、尿液样本中的ELN含量。人弹性蛋白（ELN）是一种重要的细胞外基质蛋白，赋予组织弹性，由ELN基因编码。其研究涉及其在结缔组织中的结构、功能及其在疾病如动脉粥样硬化中的作用，以及通过基因工程和重组技术制备的弹性蛋白在医学和美容领域的应用。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于体外实验中探究弹性蛋白在细胞分泌、组织重塑中的动态变化，为皮肤衰老机制、动脉弹性研究、肺功能评估等基础科研领域提供灵敏的分析工具，亦可用于药物开发过程中生物标志物的定量评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Elastin ELISA Kit; ELN ELISA Kit; ELN_HUMAN ELISA Kit; SVAS ELISA Kit; Tropoelastin ELISA Kit; WBS ELISA Kit; WS ELISA Kit
缩写：ELN
Uniprot No.：P15502
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ELN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 84 | ||||
| Range % | 80-100 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 92-112 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human ELN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 97 | 90-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.7022.9342.8182.7171002.1962.1842.1902.089501.4971.4621.4801.379250.8850.8760.8810.78012.50.4770.4860.4820.3816.250.2440.2560.2500.1493.120.1520.1610.1570.05600.1010.1000.101 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ELN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ELN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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